目的 研究酶法-超声提取白花蛇舌草(Hedyotis diffusa)总黄酮的最佳工艺。方法 以总黄酮含量为指标,通过单因素实验考查酶的种类、乙醇浓度、酶用量、酶解时间、料液比5个因素对白花蛇舌草总黄酮提取率的影响,并在此基础上通过中心组合实验设计(Central composite design, CCD)和响应面分析(Response surface methodology,RSM)优化白花蛇舌草总黄酮的提取工艺。结果 当选用纤维素-果胶-木蛋白酶,乙醇浓度为60%、酶量为0.7%、酶解时间为90 min、料液比为1∶25(g·mL-1)时,白花蛇舌草总黄酮提取率最高,为1.95%。结论 通过响应面法优选的白花蛇舌草总黄酮提取工艺合理可行,可作为提取白花蛇舌草总黄酮的一种新方法被采用。
Abstract
OBJECTIVE To optimize the extraction process of total flavonoids from Hedyotis diffusa, which have good anti-tumor effect, and improve the yield by combining enzyme extraction method and ultrasonic extraction method. METHODS Based on the total flavonoids content, the effects of enzyme type, ethanol concentration, enzyme dosage, enzymatic hydrolysis time and solid-liquid ratio on the extraction rate of total flavonoids from Hedyotis diffusa were studied by single factor experiment. Central composite experimental design (CCD) and response surface methodology (RSM) were used to optimize the extraction process. RESULTS When cellulose-pectin-wood protease was used, the ethanol concentration was 60%, the enzyme amount was 0.7%, the enzymatic hydrolysis time was 90 min, and the ratio of material to liquid was 1∶25(g·mL-1). The extraction rate of total flavonoids from Hedyotis diffusa was 1.95%. CONCLUSION The optimized extraction process of total flavonoids from Hedyotis diffusa by response surface methodology is reasonable and feasible, and the operation is simple. It can be used as a new method for extracting total flavonoids from Hedyotis diffusa.
关键词
白花蛇舌草 /
总黄酮 /
酶法 /
提取 /
响应面法
{{custom_keyword}} /
Key words
Hedyotis diffusa /
total flavonoid /
enzymatic method /
extraction /
response surface method
{{custom_keyword}} /
中图分类号：
R932
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch.P (2015) Vol Ⅰ(中国药典2015年版.一部)[S]. 2015.
[2] XU G H, KIM Y H, CHI S W, et al. Evaluation of human neutrophil elastase inhibitory effect of iridoid glycosides from Hedyotis diffusa[J]. Bioorg Med Chem Lett, 2010, 20(2):513-515.
[3] WANG Q L, XUE Y J, HAN T.The expression of CLIC4 protein in gastric cancer and the effect of total flavonoids of Hedyotis diffusa on its expression[J]. Chin J Clin Exp Pathol(临床与实验病理学杂志), 2016, 32(6):681-684.
[4] LI F, YANG P M, CAO G S, et al. Optimization of Extraction Process of Total Flavonoids from Hedyotis diffusa by Box-Behnken Design-Effect Surface Methodology[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2015, 21(8):21-24.
[5] LIU X, LI W, LI C L, et al.Study on the extraction of total flavonoids from Hedyotis diffusa by microwave assisted enzymatic method[J]. Hubei Agric Sci(湖北农业科学), 2016, 55(14):3708-3712.
[6] LI Y H, MA Y H, HUANG K H, et al. Extraction of aloe polysaccharides by cellulase assisted with ultrasonic and studies on antitumor activity[J]. J Chin Inst Food Sci Tech, 2015.15(11):91-97.
[7] SHENG Z L, WAN P F, DONG C L, et al. Optimization of total flavonoids content extracted from Flos Populi using response surface methodology[J]. Ind Crops Products, 2013, 43:778-786.
[8] HUANG R S, QIAN Y L, LIU R N, et al. Ultrasonic-assisted extraction process of total flavonoids from Zingiber mioga and its antioxidant activity[J]. Chin Pharm J(中国药学杂志), 2016, 51 (19):1652-1656 .
[9] LI B.Research progress in chemical constituents and pharmacological effects of Hedyotis diffusa[J]. Tianjin Pharm(天津药学), 2016, 28(5):75-78.
[10] LUO S Y, ZHOU L, LV X H, et al. Study on the effect and immunological mechanism of total flavones of Hedyotis diffusa on experimental ulcerative colitis[J]. China J Chin Mater Med(中国中药杂志), 2014, 39 (5):896-900.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
甘肃省自然科学基金项目资助(18JR3RA195);甘肃省中药药理与毒理学重点实验室开放基金项目资助(ZDSYS-KJ-2016-009);甘肃省高校重大疾病分子医学与中医药防治重点实验室(FZYX14-10)
{{custom_fund}}
PDF(1990 KB)
